The Signaling Adaptor p62 Is an Important NF-κB Mediator in Tumorigenesis
نویسندگان
چکیده
Supplemental Experimental Procedures Reagents and antibodies Reagents were purchased as follows: DRB, wortmannin, NAC, doxycycline, BHA, polybrene and H2O2 were from Sigma, and PD98159 from Calbiochem. Polyclonal anti-phospho-AKT, antiAKT, anti-phospho-S6K, anti-phospho-ATF2, anti-ATF2, anti-phospho-p38, anti-p38, antiphospho-IKKα/β and anti-JNK antibodies were from Cell Signalling. Anti-actin, anti-phosphoJNK, anti-IKKβ, anti-IKKγ, anti-TRAF6, anti-UBI, anti-phospho-c-Jun, and anti-c-Jun antibodies were purchased from Santa Cruz. Polyclonal anti-p62 C-ter was from Progen and human monoclonal anti-p62 was from BD. Anti-Ras was from Calbiochem. All antibodies were used according to manufacturers’ instructions.
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